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REDPROBES

About Us

redprobes

Redprobes is specialised in the development of fast and user-friendly tests for the direct detection of heparins and other sulfated polysaccharides in various matrices. We aim to provide convenient and cost-effective analytical solutions to researchers in the life sciences. The company’s proprietary, patent protected technology enables the direct measurement of heparin concentrations in µg/mL, independent of the effect on the clotting cascade. Professor Roland Krämer is a founder of Redprobes. He holds a chair in Chemistry at Heidelberg University and has published extensively on the development of molecular sensors and assays for bioanalytical and diagnostic applications.

Technology

Heparin Red®

The key component of our assays is Heparin Red®, a synthetic molecular probe that changes fluorescence intensity when associating with a polyanionic heparin target. [1] Heparin Red was designed as a fluorescent probe that emits red light, in order to reduce interference by colored components and autofluorescence in biological samples. Our technology enables the direct detection of heparins and other sulfated polysaccharides by a simple mix-and-read microplate assay, independent of any anticoagulant activity.

[1] Warttinger U, Giese C, Harenberg J, Holmer E, Krämer R. A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma. Anal. Bioanal. Chem. 2016, 408, 8241-8251.

Products

The Heparin Red® Kit has been optimised for the direct detection of heparins in human plasma. The assay actually measures “chemical” heparin concentrations in µg/mL, not the effect of heparin on the clotting cascade [1]. The Kit is emerging as a tool for pharmacokinetic studies of non-anticoagulant heparins [2], a promising class of drug candidates, and has also been reported to detect other sulphated polysaccharides.

  • convenient mix-and-read fluorescence assay for microplates
  • direct detection of heparins in only 20 µL plasma
  • quantification in the low µg/mL range
  • detection of non-anticoagulant heparins and other sulfated polysaccharides
  • robust, protein-free reagents

[1] Warttinger U, Giese C, Harenberg J, Holmer E, Krämer R. A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma. Anal. Bioanal. Chem. 2016, 408, 8241-8251.

[2] Galli M, Magen H, Einsele H, Chatterjee M, Grasso M, Specchia G, Barbieri P, Paoletti D, Pace S, Sanderson R D, Rambaldi A, Nagle A. Roneparstat (SST0001), an innovative heparanase (HPSE) inhibitor for multiple myeloma (MM) therapy: first in man study. Blood 2015, 126, 3246-3246.

Heparin Red® Ultra has been optimised for the fast and very convenient detection of heparins in complex matrices. The assay actually measures “chemical” heparin concentrations in µg/mL, not the effect of heparin on the clotting cascade. Heparin Red® Ultra was reported to suppress interference by nucleic acids and detect heparin in urine.

  • Very convenient mix-and-read fluorescene assay for microplates
  • direct detection of heparins in small sample volumes
  • quantification in the low µg/mL range
  • robust, protein-free reagents

Request a quote

The sale and supply of our products are made exclusively for research purposes and laboratory use.

Heparin Red® Kit: One Kit for about 100 or 500 determinations in 96-well microplates.

Heparin Red® Ultra: One vial for about 100 determinations in 96 well microplates.

Products may not be available for sale in all countries. Please contact us for the availability of a specific product in your country.

Email Redprobes for a quote: info@redprobes.com.

Publications

Publications related to Redprobes technology

Publications related to Redprobes technology

Redprobes is not in any way responsible for contents and accuracy of the publications listed below.

Lantero E, Aláez-Versón C R, Romero P, Sierra T, Fernàndez-Busquets X.. Repurposing Heparin as Antimalarial: Evaluation of Multiple Modifications Toward In Vivo Application  Pharmaceutics 2020, 12, 825.

Felz S, Neu T R, van Loosdrecht M C M, Lin Y. Aerobic granular sludge contains Hyaluronic acid-like and sulfated glycosaminoglycans-like polymers. Water Research 2020 , 169, 115291.

Gezelius E, Bendahl P O,  Goncalves de Oliveira K,  Ek L, Bergman B, Sundberg J, Strandberg K, Krämer R, Belting M. Low-molecular-weight heparin adherence and effects on survival within a randomised phase III lung cancer trial (RASTEN). Eur. J. Cancer 2019, 118, 82-90.

Zayed A, Dienemann C, Giese C, Krämer R, Ulber R. An immobilized perylene diimide derivative for fucoidan purification from a crude brown algae extract. Process Biochem., 2018. 65, 233 – 238.

Rappold U, Warttinger U, Krämer R. A Fluorescent Probe for Glycosaminoglycans Applied to the Detection of Dermatan Sulfate by a Mix-and-Read Assay. Molecules 2017, 22, 768. 

Warttinger U, Giese C, Harenberg J, Holmer E, Krämer R. A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma. Anal. Bioanal. Chem. 2016, 408, 8241-8251.

Galli M, Magen H, Einsele H, Chatterjee M, Grasso M, Specchia G, Barbieri P, Paoletti D, Pace S, Sanderson R D, Rambaldi A, Nagle A. Roneparstat (SST0001), an innovative heparanase (HPSE) inhibitor for multiple myeloma (MM) therapy: first in man study. Blood 2015, 126, 3246-3246.

Szelke H, Schuebel S, Harenberg J, Kraemer, R. A fluorescent probe for the quantification of heparin in clinical samples with minimal matrix interference. Chem Commun. 2010, 46, 1667-1669.

Szelke H, Schuebel S, Harenberg J, Kraemer, R. Interaction of heparin with cationic molecular probes: Probe charge is a major determinant of binding stoichiometry and affinity. Bioorg. Med. Chem. Lett. 2010, 20, 1445-1447.

Contact

eMail: info@redprobes.com

Impressum

Redprobes UG (haftungsbeschränkt)
Haxthausenweg 50
48165 Münster
Deutschland


E-Mail: info@redprobes.com
Internet: redprobes.com
Redprobes UG (haftungsbeschränkt) wird vertreten durch die persönlich haftende Gesellschafterin:
Redprobes UG (haftungsbeschränkt), AG Münster, HRB 14676;
diese wird vertreten durch: Geschäftsführerin Claudia Krämer

Registergericht: Amtsgericht Münster
Handelsregister Registernummer: HRB 14676

Umsatzsteuer-Identifikationsnummer gemäß § 27a Umsatzsteuergesetz (UStG): DE292523367

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